Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli
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چکیده
منابع مشابه
Enzymatic Basis of Hygromycin B Resistance in Escherichia coli
A plasmid conferring resistance to the aminocyclitol antibiotic hygromycin B was isolated from Escherichia coli. The gene conferring resistance to this drug was cloned in pBR322, and the gene was localized to a fragment of ca. 1,510 base pairs. Resistance to hygromycin B is determined by an aminocyclitol phosphotransferase that modifies hygromycin B and structurally related antibiotics. The spe...
متن کاملHygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli.
The aminoglycoside antibiotic hygromycin B was examined in Escherichia coli cells for inhibitory effects on translation and ribosomal-subunit formation. Pulse-chase labeling experiments were performed, which verified lower rates of ribosomal-subunit synthesis in drug-treated cells. Hygromycin B exhibited a concentration-dependent inhibitory effect on viable-cell numbers, growth rate, protein sy...
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Antibiotic producing-organisms generally exhibit resistance to the antibiotics that they produce1). Such resistance mechanisms may take the form of target site modification, as is the case with erythromycin2) and thiostrepton3), or enzymatic modification of the antibiotic itself, as has been shown for a number of aminocyclitols4) The existence of these resistance mechanisms in producing organis...
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Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by Streptomyces hygroscopicus. Ribosomes from this Gram-positive mycelial bacterium are inhibited in vitro by the antibiotic. In contrast, the streptomycete is highly resistant to the drug in vivo since it possesses hygromycin B phosphotransferase activity. This enzyme has been shown by g...
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Bacterial populations have a remarkable capacity to cope with extreme environmental fluctuations in their natural environments. In certain cases, adaptation to one stressful environment provides a fitness advantage when cells are exposed to a second stressor, a phenomenon that has been coined as cross-stress protection. A tantalizing question in bacterial physiology is how the cross-stress beha...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1983
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.24.5.689